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KMID : 0352720080320040291
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Journal of Ginseng Research
2008 Volume.32 No. 4 p.291 ~ p.299
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Identification of NMR Data for ginsenoside Rg1
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Lee Dae-Young
Cho Jin-Gyeong
Lee Min-Kyung
Lee Jae-Woong
Park Hee-Jung
Lee Youn-Hyung
Yang Deok-Chun
Baek Nam-In
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Abstract
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The fresh ginseng roots were extracted in aqueous methanol (MeOH), and the obtained extracts were partitioned using ethyl acetate (EtOA), n-butanol (n-BuOH), and water, successively. The repeated silica gel column chromatography
for n-BuOH fraction afforded a purified ginsenoside Rg1. The physico-chemical, spectroscopic and chromatographic data
of ginsenoside Rg1, such as crystallization characteristics, melting point, specific rotation, infrared spectrometry (IR) data,
fast atom bombardment/mass spectrometry (FAB/MS) data, nuclear magnetic resonance (NMR) data, retention factor
(Rf) in thin layer chromatography (TLC) experiment, and retention time (r.t.) in HPLC analysis, were measured and compared with those reported in literatures. Especially, the previous literatures reported different data for ginsenoside Rg1 in the 1H- and 13C-NMR experiments. This paper gives the exactly assigned NMR data through 2D-NMR experiments, such as 1H-1H correlation spectroscopy (COSY), hetero nuclear single quantum correlation (HSQC), and hetero nuclear multiple bond connectivity (HMBC).
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KEYWORD
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ginsenoside Rg1, NMR, HMBC, melting point, specific rotation, FAB/MS, IR, TLC, HPLC
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